Protein Synthesis in Bupivacaine Skeletal Muscle (marcaine)-treated, Regenerating
نویسنده
چکیده
myotoxic drug bupivacaine (Marcaine) into the rat tibialis anterior muscle. Doses of 1.5 and 1 .O% wt/vol produce significant levels of muscle regeneration, but these doses also produce large regions of ischemic muscle. Doses of 0.75 and 0.5% bupivacaine are also effective in inducing regeneration and produce little or no ischemia. Regenerating muscle is significantly more active in the incorporation of 35S-methionine into protein than is control muscle, and the activity increase is directly proportional to the bupivacaine dose injected. Polyribosomes were isolated in greeter yield from bupivacaine-treated muscles, as compared with control muscles, 5 days postinjection, and were also more active in cell-free protein synthesis than control polysomes. Again, the yield and activity of the muscle polysomes was directly proportional to the bupivacaine concentration used for injection. Polyacrylamide gel electrophoresis of polysomal cellfree reaction mixtures demonstrated the synthesis of a number of myofibrillar proteins.
منابع مشابه
Effects of marcaine, a myotoxic drug, on macromolecular synthesis in muscle.
--The effects of Marcaine (bupivacaine) on RNA and protein synthesis in skeletal muscle have been studied. The drug did not affect RNA synthesis by pieces of rat tibialis anterior at concentrations as high as 0.5% (w/v), nor did it affect cell-free transcription of calf thymus DNA by wheat germ RNA polymerase I!. In contrast, Marcaine inhibited protein synthesis by muscle chunks, and also inhib...
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